Under the current period background of big data and cloud computing, there is a huge demand for professionals in related fields such as information technology (IT). To solve this problem, this paper puts forward an IT professional talents training model based on animal cell structure by comparing the structures of animal cells and its efficient operation principle with IT professional training model system. According to the efficient-working principle of ‘Nucleus-Cytoplasm- Environment’, this model is built as a ‘Class (The Core)-College (Internal Environment)-Enterprise (External Environment)’ training model for IT-majored students. The motivation is to cultivate students’ abilities in these four aspects: structure, application, analysis and innovation, namely, regarding theory teaching as the core, college practice training as the pulling force and enterprise project resources as the pushing force. The reliability and validation of this model have been demonstrated by simulation results in Wuhan University of Science and Technology.