Gaming simulation is a form of experiential learning in a gaming environment. In this paper, we are interested in the use of gaming simulation in IS Security teaching in a university laboratory setting. The students enrolled in this course are business students from different business disciplines, with diverse IS/IT knowledge. This study reports on our recent experience at using a sophisticated gaming simulation software (CyberCIEGE) in a IS Security course. We explored whether students’ IS Security knowledge could improve with students who have different levels of prior IT knowledge. The design of the study is one group pre-test-post-test pre-experimental. We conclude the paper with a recommendation on the suitability of the software for future use, a discussion of its limitations and suggestions for extending this research.