Issues concerning global virtual collaboration have received considerable attention in IS research and practice; however, little research has been conducted on knowledge-sharing activities in global virtual collaboration, which is a key process to achieve collaboration effectiveness. In this paper, we investigate the impact of national culture on knowledge-sharing activities in global virtual collaboration from dynamic and individual perspectives. An exploratory case study was first used to explore how national culture impacts knowledge-sharing activities in global virtual collaboration. The purpose of the case study was to understand qualitatively the phenomenon under investigation and generate a set of hypotheses. In phase two, an online survey was used to test the hypotheses. The goal was to refine and generalize the findings from the first phase. The results emphasize the role of non-value-based cultural dimensions in global virtual collaboration and the importance of investigating national culture from an individual level and in practice.