Based on the supply chain collaboration literature and electronic collaboration literature, this paper introduces the term ‘electronic supply chain collaboration’. It further introduces and studies the role that an organization’s collaboration climate plays in the perception of the impact of e-marketplace participation and adoption of electronic supply chain collaboration in parallel to external pressures. While the role of external pressures has been extensively studied in the pertinent literature, the role of company collaborative climate-culture has been rather ignored in this context, despite the fact that focus groups with experts within the framework of the presented research identified culture as a key determinant. A field survey is conducted with 81 respondents among users of electronic supply chain collaboration services through e-marketplaces in order to identify the effect of collaborative climate and the role of the perception of the impact of e-marketplace participation, using Institutional pressures as a “control variable” for the validity of this work. The first results indicate that the collaborative climate construct explains an organization’s intention to adopt electronic collaboration linkages that require human intervention and collaboration. It is suggested that e-collaboration climate, based on culture theory, should be included in the research as a formative construct composed by internal collaboration, technology orientation, innovativeness and other cultural concepts. Further research is required in order to better understand these findings.