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Lithuania has a well-developed transportation system. Its geographically strategic position is very important to European transport corridors. In Lithuania, there are various important factors that contribute to shaping the sector’s characteristics — lack of knowledge, a low level of computerization and Internet usage – that cause ’ebusiness possibilities’ not to be used. An appropriate analysis of effective e-business usage for the development of competitive advantage between transportation companies is a very important task which requires theoretical grounding and research of practical possibilities. The purpose of paper is to analyze the use of e-business in Lithuania’s transportation companies. The survey was supposed to act as a guideline to the future of Internet usage in the Lithuanian transportation industry. The survey tried to find out considerations about the Internet, how it had affected their business and activities and what might happen in the future.