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How do we develop and sustain trust? What is the process for building trust between business partners in virtual environments? Is there a significant difference between the development and sustainability of trust online or offline? In this paper, we first introduce the concept of e-business and discuss the importance of trust for ensuring effective collaboration. Secondly, we discuss the relationships between e-collaboration and trust for managing e-business. Thirdly, we suggest a framework, which may help facilitate the development and sustainability of trust in an online environment. Finally, implications for the development and sustainability of trust online, which can be used to understand the interplay among technologies, e-business and collaboration is provided. We suggest that the implications of this study are three-fold: trustworthy relationships among business partners, effective sustainable collaboration, and optimal use of ICT for supporting e-business activities.