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Organizational culture is an important factor in organization development process. This paper explores the culture issue in the new technology age. Adopting Ruppel’s new culture framework developed from traditional perspective on culture the paper tested the culture difference between organization with intranet and without intranet. Using Confirmatory Factor Analysis method with Lisrel the paper explored the research area on culture and extended the usage of Lisrel into MIS area. The result showed that organizational culture in the traditional and E-commerce Company is different in the ethical value. The research result showed that the culture is changing in the process of adopting new technology in organization, which may raise the attention of changing the management methods due to differences in culture.