In an increasingly connected and digital world, information is seen as a business enabler and source of sustained competitive advantage. Thus, information security is becoming critical to protect these information assets, which is why organizations’ information security strategy has been aligning with their strategic goals. This paper aims to study organizations’ general information security environment, analyse the CISO’s role in them and understand where they should be positioned on the organizational structure. Interviews were conducted on experienced information security consultants, information systems and information security directors, which allowed to conclude that organizations in Portugal still need to increase their maturity when it comes to information security, and that this may be due to the absence of an established security culture in the country. On the other hand, the CISO’s role has been increasing in relevance, being considered that it should have a close and independent relationship with organizations’ boards.