This study presents a conceptual model to investigate the e-health readiness required by healthcare organization and the impact of such readiness in enhancing e-health transformation success. A scale to measure e-health readiness was developed and validated; then it was used to collect the large-scale survey data. The partial least squares (PLS) method was used to empirically test the conceptual model and hypotheses through the large-scale survey collected. The empirical results support the proposed model. The results provide a means to understand what factors determine the e-health transformation success and can potentially be used in advance of e-health transformation for contemporary hospitals to check its e-health readiness level and prepare the needed changes for e-health implementations to ensure better e-health transformation.