This research is based on a qualitative approach with in-depth case study using Nonaka’s theory of knowledge creation. It addresses the high-level need to make collaboration more effective between university and NGO by exploring knowledge sharing challenges. It used semi-structured interviews, and project documentation to look at the ways in which researchers and NGO representatives with different culture within University-NGO partnerships share information and knowledge. The study will propose guidelines that reflect five key steps which are important in order to show how collaborative projects can build a successful knowledge sharing environment.