The study of ambidexterity continues to attract attention from scholars across multiple business domains. This has contributed to several reviews of the ambidexterity field being conducted and reported in the recent past. This article builds on the existing reviews and takes stock of the ambidexterity research, with a focus on Information Systems (IS) domain. Furthermore, this systematic review takes a unified perspective of the field and addresses some of the ongoing debates about the conceptualisation of the ambidexterity construct, trade-offs being addressed as well as its relevance and applicability for IS research.