Drawing on Cameron and Quinn’s organisational cultures typology that defines four types of organisational culture (i.e., clan, adhocracy, market, and hierarchy), and Daniel et al.’s four-stage model of e-commerce adoption, this paper empirically examines the influence of different organisational cultures on e-commerce adoption maturity. The result indicates a positive correlation between adhocracy culture and e-commerce adoption. However, those firms with hierarchy cultural characteristics indicate a negative correlation in relation to e-commerce adoption. The organisational culture differences explain these issues. This research has a number of implications to e-commerce stakeholders.