The main objective of this study is to examine gender differences and technology acceptances from tertiary education students from Chile. The TAM model is the tool used to measure the acceptance and use of e-learning of respondents. Partial Least Squares (PLS) was used, specifically, multi-group PLS analysis was used to compare between-group differences. In summary, we can affirm that in a sample of tertiary Chilean students their behavior of acceptance of e-learning technology match with to TAM model and it don’t shows significant statistic differences between women and men.