Based on the evolutionary game theory and the bounded rational and asymmetric information assumptions, we construct the dynamic evolutionary game model between the three game players and analyze the replication dynamic equations. Furthermore, we analyze the strategy of three symbiosis units and the evolution mechanism of E-commerce industrial park symbiosis system. Finally, two conclusions are drawn: Conclusion 1, the probability that three symbiosis units of E-commerce Industrial Park choosing the symbiosis strategy increases with increase of the probability that the other two symbiosis units selecting the symbiosis strategy. Conclusion 2, the probability of the symbiosis unit to choose the symbiosis strategy increase with the increase of the benefit and decrease with the increase of the cost, risk and loss.