Healthcare is regarded as one of the fundamental rights of every human. Healthcare organizations (HCO) are making great efforts to provide to their customers the best possible healthcare services therefore HCO need to constantly view knowledge as one of the organization's strategic asset. Knowledge in HCOs need to be discovered, created, shared and applied. However, the studies on KM implementation in HCO is deemed insufficient. The aim of this study is to investigate the perception of healthcare professionals (HCP) on KM practices in HCO in Malaysia. The investigation includes the HCPs perceived benefits, supporting factors and challenges in implementing KM in their organization. This study adopts a case study research design. Questionnaires were distributed online to HCPs of a HCO. The findings indicate that HCPs were aware of the KM but not convinced with its capabilities and the benefit it could bring to the organization.