Contemporary organizations recognize the importance of green innovation for their competitive advantages in the long run, and most existing studies focus on its impacts on organizational performances. Based on the resource-based view, this study examines the roles that green information systems (IS) infrastructure and green culture play in green innovation as tangible and intangible resources. In addition to their direct impacts, green IS infrastructure and green culture may have indirect relationships with green innovation through the alignment between two as well as their alignments with green innovation. The hypothesized triadic relationships among green IS infrastructure, green culture and green innovation effectiveness were tested with survey observations collected from organizations in China. The statistical analyses supported all the direct relationships as hypothesized, but yielded mixed results on the mediating relationships. The alignment between GIS and green innovation had positive effect, the alignment between green IS and green culture had insignificant effect, and the alignment between green culture and green innovation had negative effect. In addition, each aspect of alignment played somewhat different roles across organizations of different sizes.