This study makes an attempt to extend technology acceptance model (TAM) and presents a respecified conceptual model to examine the factors associated with college students’ use of asynchronous e-learning systems. A web-based learning platform was employed to assist the learning of an undergraduate-level course, management information systems (MIS), in a well-known institute of technology in the southern part of Taiwan. A cross-sectional survey was conducted. The partial least squares method was applied to validate the reliability and validity of the measurement model and assess the proposed conceptual model in this study. The empirical results indicated that college students showed great readiness and positive intentions towards the use of such e-learning system for the professional courses and suggested potential benefits from its use in the long term. The findings of this study not only can proffer practical implications for on-line professional course learning and teaching in business education, but also may serve as instrumental guidelines for e-learning system to be designed effectively to improve students’ interests and motivations in virtual learning environments.