Thanks to the surge of health information technology (HIT) investment, hospitals have evolved into an IT-intensive industry. Hospital nurses and physicians spend much time working with computer-based clinic application systems in their daily undertaking of health care service, and the effective use of HIT becomes critical to the success of hospitals. Behavioral research on the adoption of HIT lags behind the fast proliferation of the technology. Many researchers frame HIT adoption with theories that have been validated in other industries without examining the special context of hospital operations. This research attempts to study the influence of hospital IT environment and personal IT knowledge on people’s reactions to HIT systems. A research model was developed, and a field study at two midsized hospitals was conducted to test the hypothesized relationships. Implications of the results for both HIT researchers and practitioners are discussed.