Building from organizational climate theory, recent conceptual work has extended the IT service quality research and proposed a new construct, IT Service Climate, as an antecedent of IT service quality (Jia and Reich 2008). This paper reports on an empirical study that validated a 14-item measurement instrument for the IT service climate construct. Consisting of three dimensions, including Service Leadership, Service Vision, and Service Evaluation, the instrument demonstrates satisfactory reliability, convergent validity/unidimensionality, and discriminant validity. Data from a sample of matched pairs of systems and client units also indicate that, service climate scores from systems units explained significant variance in IT service quality as rated by their respective client units, thus demonstrating criterion validity. Contributions to both IT research and practice are discussed.