The contribution of this paper is a methodology for profiling literature in Information Systems (IS) using a powerful tool for co-citation analysis - Citespace. Co-citation analysis provide important insights into knowledge domains by identifying frequently co-cited papers, authors and journals. The methodology is applied to a dataset comprising of citation data pertaining to a leading European journal – the European Journal of Information Systems (EJIS). In this paper we outline the different steps involved in using Citespace to profile literature in IS and use the EJIS dataset as an example. We hope that the readers will employ and/or extend the given methodology to conduct similar bibliometric studies in IS and other research areas.