Innovative work behavior is crucial to fulfill the demands for a lecturer’s research activities especially at research universities. Prior literature indicated that psychological empowerment is an important predictor for innovative work behavior. Meanwhile, Web 2.0 is an indispensable tool for innovation among lecturers to facilitate their research activities. Thus, social cognitive theory was used to support the conceptual framework in identifying the mediating role of Web 2.0 usage in the effect of psychological empowerment on innovative work behavior. This study utilized quantitative approach using questionnaire as a tool to gather data from 393 lecturers of five research universities in Malaysia. Structural Equation Modeling (SEM) was used to analyze the direct and mediation effects of the variables in the conceptual framework. Web 2.0 usage was identified as the mediator for the effect of psychological empowerment on the realization of new ideas in innovative work behavior, specifically for the dimensions of meaning, competence and self-determination. Based on the results of the study, use of Web 2.0 is important among lecturers to enhance innovative work behavior.